Invitrogen™ CD204 Monoclonal Antibody (2F8)

Rat Monoclonal Antibody

Brand:  Invitrogen™ MA516494

Product Code. 15205799

Description

In Western blot, this product recognizes CD204 in J774 cells under non-reducing conditions only. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.

CD204 gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Diseases associated with MSR1 include Barrett Esophagus and Prostate Cancer.

Specifications

• Antigen: CD204
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P30204
• Gene Symbols: MSR1
• Immunogen: RAW264 cell line.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Pig, Fish
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Clone: 2F8
• Conjugate: Unconjugated
• Gene: MSR1
• Gene Alias: bovine scavenger receptor type II; CD204; Macrophage acetylated LDL receptor I and II; macrophage scavenger receptor 1; macrophage scavenger receptor type III; macrophage scavenger receptor types I and II; MRS-A; MSR; Msr1; MSR-A; phSR1; phSR2; RGD1564316; SCARA1; Scavenger receptor class A member 1; scavenger receptor class A, member 1; scavenger receptor type A; scavenger receptor type I; Scvr; SRA; SR-A; SR-AI; SR-AI/II; SR-AII
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100511665, 20288
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid