CD158A Mouse anti-Human, Brilliant Violet 711, Clone: HP-3E4, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 563183

Product Code. 15892579

Description

The HP-3E4 monoclonal antibody reacts with CD158a. CD158a is a 58 kDa membrane glycoprotein, member of the killer-inhibitory receptor (KIR). The KIR family family consists of transmembrane glycoproteins of the Ig superfamily expressed on <1-8% of NK cells and a subset of T cells which are involved in recognition of MHC class I molecules on target cells and inhibit cytotoxicity. CD158a is an antigen which recognizes HLA-C molecules (-Cw*0401 and -Cw*1503). This antibody is suitable for staining acetone-fixed, frozen tissue sections. It was reported that this clone also crossreacted with NKAT8 (CD158i) tranfectant cells.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

• Antigen: CD158a
• Classification: Monoclonal
• Conjugate: Brilliant Violet 711
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Human NK Cells
• Quantity: 50 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: HP-3E4
• Description: KIR2DL1; KI2L1; NKAT; NKAT-1; NKAT1; KIR-K64; KIR221; p58.1
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgM