Caveolin 2 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA1065

Product Code. 11561472

Description

PA1-065 detects caveolin-2 from rat and mouse tissues tissues. This antibody does not detect caveolin-1 or -3. PA1-065 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a ∽21 kDa protein representing caveolin-2 from rat heart homogenate. The PA1-065 immunogen is a synthetic peptide corresponding to residues M(1) G L E T E K A D V Q L F M A D D A Y(19) C of rat CAV2. This sequence is completely conserved in mouse, and ∽90% conserved in human caveolin-2. The PA1-065 immunizing peptide (Cat. # PEP-080) is available for use in neutralization and control experiments.

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly

Specifications

• Antigen: Caveolin 2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CAV2
• Gene Alias: AI447843; CAV; CAV2; caveolae; caveolae protein, 20-kD; caveolin 2; caveolin 2 isoform a and b; caveolin-2; MGC12294; Phospho-CAV2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12390, 363425
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: Q2IBC5, Q9WVC3
• Gene Symbols: CAV2
• Immunogen: Synthetic peptide corresponding to residues M(1) G L E T E K A D V Q L F M A D D A Y(19) C of rat CAV2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG