Caspase 9 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA516358

Product Code. 11355763

Description

PA5-16358 targets Caspase 9 in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Bovine, Human, mouse, Ovine, and Rat samples. The PA5-16358 immunogen is recombit protein encoding aa 1-134 of human caspase 9.

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer’s Disease (AD).

Specifications

• Antigen: Caspase 9
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CASP9
• Gene Alias: CASP-9, ICE-like apoptotic protease 6,apoptotic protease MCH-6, apoptotic protease activating factor 3, apoptotic protease-activating factor 3, caspase 9, apoptosis-related cysteine protease, caspase-9, cas9
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100140945, 101110953, 12371, 58918, 842
• Content And Storage: 4° C
• Form: Liquid

• Applications: Hemagglutination Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
• Gene Accession No.: P55211, Q8C3Q9
• Gene Symbols: CASP9
• Immunogen: Recombit protein encoding aa 1-134 of human caspase 9
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Human, Mouse, Ovine, Rat
• Product Type: Antibody
• Isotype: IgG