c-Cbl (pY774) Mouse anti-Human, Unlabeled, Clone: 29/C-CBL, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 558035

Product Code. 15869168

Description

Cbl (Casitas-lineage lymphoma) was identified in the genome of a transforming retrovirus from a mouse pre-B lymphoma. The cellular gene product c-Cbl is one of numerous Cbl-related proteins found in vertebrate and invertebrate organisms. It is an 120-kDa adapter protein that contains multiple functional domains, including a RING finger motif, a tyrosine kinase-binding (TKB) domain, and a proline-rich region. The TKB domain directly interacts with specific auto-phosphorylation sites in activated protein-tyrosine kinases (PTK). Through the RING finger motif, c-Cbl recruits and activates an E2 ubiquitin-conjugating enzyme, thus targeting the activated PTK for protein degradation. The proline-rich region contains SH3 domain-binding and 14-3-3 protein-binding motifs. c-Cbl is also phosphorylated at tyrosines 700, 731, and 774 (Y774) by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for antigens, immunoglobulins, growth factors, cytokines, and hormones. In turn, the phosphorylated Y774 site interacts with the SH2 domain of the CRK adapter protein. The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells. Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses.

The 29/c-Cbl monoclonal antibody recognizes the Y774-phosphorylated form of human c-Cbl.

Specifications

• Antigen: c-Cbl (pY774)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human c-Cbl Peptide
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Western Blot
• Clone: 29/C-CBL
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.