BMI-1 Mouse anti-Human, Alexa Fluor 488, Clone: P51-311, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 562636

Product Code. 15852099

Description

The P51-311 monoclonal antibody binds to human BMI-1 (B lymphoma Mo-MLV insertion region 1 homolog). BMI1 is a c-myc cooperating oncogene that encodes an ∽45 kDa protein that is a member of the Polycomb Group (PcG) of proteins. PcG proteins are essential for the maintenance, but not initiation, of the transcriptionally repressed state of certain developmental genes. PcG proteins are a structurally diverse group of proteins with conserved functions from fly to human cells. PcG proteins form complexes and regulate the expression of genes involved in cell cycle, DNA repair and differentiation that are crucial for maintaining the self renewal of normal and cancer stem cells. Specifically, BMI-1 is a core component of PRC1 (polycomb repressive complex 1). BMI-1, via the up-regulation of hTERT and independent of c-myc, can immortalize mammary epithelial cells. BMI-1 has also been shown to repress the INK4A locus that controls the tumor suppressors p16 and p19ARF (mouse homologue of p14ARF) in mouse models. BMI-1 plays a role in maintaining the self-renewal capacities of stem cells including hematopoietic, intestinal, retinal and neural stem cells. During antibody development, the purified P51-311 monoclonal antibody was found to detect BMI-1 by Western blot analysis of cellular lysates and by indirect immunofluorescent staining and flow cytometric analysis of fixed and permeabilized cells.

Specifications

• Antigen: BMI-1
• Classification: Monoclonal
• Conjugate: Alexa Fluor 488
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
• Immunogen: Human BMI-1 Recombinant Protein
• Quantity: 50 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: P51-311
• Description: BMI1; PCGF4; polycomb group ring finger 4; RING finger protein 51; RNF51
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ