Aurora A Mouse anti-Human, Mouse, Clone: 35C1, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA180399

Product Code. 11555452

Description

Clone 35C1 specifically recognizes an epitope within the non-catalytic N-terminal domain of Aurora-A, and does not inhibit Aurora-A kinase activity. A suggested positive control for immunohistochemical applications is human 293 and mouse llc1 cell lines. In Western blot, this antibody detects a band at ∽46kDa in human HeLa and mouse M-ICc12 cell lysates.

Aurora A is a 46 kDa protein that plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment. Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Multiple transcript variants encoding the same protein have been found for this gene. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal.

Specifications

• Antigen: Aurora A
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: O14965, P97477
• Gene Symbols: AURKA
• Immunogen: Recombit Aurora A
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Clone: 35C1
• Conjugate: Unconjugated
• Gene: AURKA
• Gene Alias: AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 20878, 6790
• Content And Storage: 4°C or -20°C if preferred
• Form: Liquid