Invitrogen™ CRYAB Polyclonal Antibody

Product Code.	Quantity	unitSize
11561812	100 μL	100µL

Product Code. 11561812 Supplier Invitrogen™ Supplier No. PA116950

Description

Rabbit Polyclonal Antibody

This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes. Suggested positive control: human and mouse skeletal muscle lysate, human skeletal muscle protein, antigen standard for HSPB1 (transient overexpression lysate), mouse skeletal muscle protein. Predicted to react with rat, sheep, rabbit, monkey, guinea pig, chicken, and pig based on 100% sequence homology. Immunogen sequence has 81% homology with Zebrafish.

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.

Specifications

• Antigen: CRYAB
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1.08 mg/mL
• Conjugate: Unconjugated
• Formulation: Phosphate, tris citrate with 0.1% sodium azide; pH 7-8
• Gene: Cryab
• Gene Accession No.: P02511, P23927, P23928
• Gene Alias: 2310001E10Rik; 3526402H21Rik; AACRYA; active transcription factor CREB; alpha B-crystallin; alpha crystallin B; alpha(B)-crystallin; alpha-B-crystallin; Alpha-crystallin B chain; alpha-crystallin B chain-like protein; AV083133; cAMP response element binding protein 1; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CMD1II; CREB; CREB1; CREB-1; Crya2; Crya-2; CRYAB; CryAB antibody; crystallin alpha B; crystallin, alpha 2; crystallin, alpha B; crystallin, alpha polypeptide 2; CTPP2; CTRCT16; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-responsive element-binding protein 1; epididymis secretory protein Li 101; heat shock protein beta-5; heat shock protein CryAB; heat shock protein HspB5; heat-shock 20 kD like-protein; HEL-S-101; HSPB5; HspB5 protein; MFM2; P23; Renal carcinoma antigen NY-REN-27; rosenthal fiber component; transactivator protein; Y protein
• Gene Symbols: Cryab
• Host Species: Rabbit
• Immunogen: A synthetic peptide made to an internal region of the human Crystallin AB protein (within residues 70-140).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12955, 1410, 25420
• Target Species: Human, Mouse, Rat
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG