Actin Polyclonal Antibody, Invitrogen™

Actin Antibody, PA1-16889, from Invitrogen™. Species Reactivity: Human, Mouse; Applications: Western Blot.

Overview
Brand: Invitrogen

Manufacturer Part Number: PA1-16889

ACTIN

UNSPSC: 12352203

Code: LD

Additional Details:
Additional Details: Weight: 1.24740kg



Product Code. 11581782

Quantity Price
1 £ 335.0 / 100µL
Estimated Shipment date
from Supplier 20-12-2016
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Description and Specification

Specification

Antigen Actin
Applications Western Blotting
Conjugate Unlabeled
Cross Reactivity Human
Cross Reactivity Mouse
Format Purified
Formulation 100μg purified antibody (1mg/mL) in tris citrate and 0.09% sodium azide; pH 7-8
Gene Accession No. P60709, P60710
Gene Alias beta actin, gamma actin, cytoskeleton actin, Atcb
Gene Symbols ACTB
Host Species Rabbit
Immunogen N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709 and is highly conserved with gamma actin
Isotype IgG
Quantity 100μg
Species Reactivity Human, Mouse
Storage Requirements Store at 4° C
Primary or Secondary Primary
Monoclonal or Polyclonal Polyclonal
Gene ID (Entrez) 11461, 60

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma, have been identified. The alpha actins are found in muscle tissues and are a major constituent of contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are hightly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.
Host Species: Rabbit
Cross Reactivity: Human, Mouse
Isotype: IgG
Immunogen: N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709 and is highly conserved with gamma actin
Formulation: 100μg purified antibody (1mg/mL) in tris citrate and 0.09% sodium azide; pH 7-8
Synonyms: beta actin, gamma actin, cytoskeleton actin, Atcb
Uniprot Accession: P60709, P60710
Entrez GeneID: 11461, 60
Gene Symbol: ACTB
This antibody will not work in ICC/IF applications. Suggested positive control: Hela whole cell extract.
References:
Test: WB, Dilution: 1:2000, Species: Ms, Reference: DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes. (2011 Apr) J Lipid Res, Vol: 52, 4, 657-67, Harris CA, Haas JT, Streeper RS, Stone SJ, Kumari M, Yang K, Han X, Brownell N, Gross RW, Zechner R, Farese RV Jr; Pubmed ID: 21317108