Invitrogen™ ANKRD27 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5106948

Product Code. 17186753

Description

Sequence homology: Cow: 77%; Dog: 93%; Guinea Pig: 85%; Horse: 86%; Human: 100%; Mouse: 91%; Rabbit: 93%.

May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (PubMed:16525121, PubMed:18477474). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (PubMed:23104059). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7. Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38. Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1 (PubMed:22705394). Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (PubMed:19745841). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (PubMed:24856514). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes. [UniProt].

Specifications

• Antigen: ANKRD27
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ANKRD27
• Gene Alias: ANKRD27; ankyrin repeat domain 27; ankyrin repeat domain 27 (VPS9 domain); ankyrin repeat domain-containing protein 27; PP12899; VARP; Vps9 domain and ankyrin-repeat-containing protein; VPS9 domain-containing protein; VPS9-ankyrin-repeat protein
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 84079
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 2% sucrose and 0.09% sodium azide; pH 7.4
• Gene Accession No.: Q96NW4
• Gene Symbols: ANKRD27
• Immunogen: Synthetic peptide directed towards the C-terminal region of human ANKRD27.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG