Invitrogen™ Androgen Receptor Monoclonal Antibody (AN1-15)

Rat Monoclonal Antibody

Brand:  Invitrogen™ MA1150

Product Code. 11548243

Description

MA1-150 detects androgen receptor from human, Rhesus monkey, rat and mouse tissues. This product does not cross-react with estrogen, progesterone, or glucocorticoid receptors. MA1-150 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry, gel shift, and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing androgen receptor in rat tissues. Immunohistochemical staining of androgen receptor in primate hypothalamus with MA1-150 results in intense nuclear staining. The MA1-150 immunogen is a fusion protein corresponding to residues 331-572 of human androgen receptor. Reconstitute with PBS.

The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors. The receptor is more than 90 kDa and has three major functional domains: the N-terminal domain, DNA-binding domain, and the androgen-binding domain. The androgen receptor is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT). Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis.AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain.Androgen Receptor is a phosphoprotein, and also regulates mitogen-activated protein kinase (MAP kinase). The inhibition of the MEK1/2 pathway correlates directly with a change in phosphorylation state of the androgen receptor. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease, and male infertility. Mutations in this gene are associated with complete androgen insensitivity (CAIS).

Specifications

• Antigen: Androgen Receptor
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: P10275, P15207, P19091
• Gene Symbols: AR
• Immunogen: A fusion protein corresponding to residues 331-572 of human androgen receptor.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Monkey, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Gel Shift, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: AN1-15
• Conjugate: Unconjugated
• Gene: AR
• Gene Alias: AIS; Andr; androgen nuclear receptor variant 2; androgen receptor; androgen receptor (Testicular feminization), same as Tfm; androgen receptor splice variant 4b; Ar; AR8; AR-A; AW320017; DHTR; dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; Nuclear receptor subfamily 3 group C member 4; RP11-383C12.1; SBMA; SMAX1; TFM; Variant AR45
• Host Species: Rat
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 11835, 24208, 367
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized