Invitrogen™ AMPK alpha-2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA521494

Product Code. 13268908

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3. Predicted reactivity: Mouse (98%), Rat (98%), Pig (99%), Chicken (96%), Sheep (98%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Specifications

• Antigen: AMPK alpha-2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PRKAA2
• Gene Alias: 2310008I11Rik; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; A830082D05; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP-activated protein kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMPK; AMPK alpha 2; AMPK alpha-2 chain; AMPK subunit alpha-2; AMPK2; AMPKa2; AMPKalpha2; AMPK-alpha-2 chain; EC 2.7.11.1; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; PRKAA; Prkaa2; protein kinase AMP-activated catalytic subunit alpha 2; protein kinase, AMP-activated, alpha 2 catalytic subunit
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 108079, 5563, 78975
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.96 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P54646, Q09137, Q8BRK8
• Gene Symbols: PRKAA2
• Immunogen: Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG