Invitrogen™ Human AMPK alpha-1/beta-2/gamma-3, GST Tag, His Tag Recombinant Protein

Product Code. 15920273

Brand: Invitrogen™ A30485

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. This AMPK (A1/B2/G3) recombinant human protein (full length) was expressed in insect cells. AMPK (A1/B2/G3) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service. Validated for use with the Z'-LYTE™ assay Available in multiple pack sizes for convenient use Also available in our SelectScreen Kinase Profiling Service Protein Form: Full Length, Recombinant, Active.

AMPK (AMP-activated protein kinase) serine/threonine kinase is a heterotrimeric complex, which acts as sensor of cellular energy levels. The signaling cascades initiated by activating AMPK are critical to regulation of metabolic events in the liver, skeletal muscle, heart, adipose tissue, and pancreas. This AMPK consists of the alpha1, beta2 and gamma3 subunits. While the purified AMPK (A1/B2/G3) complex can be used in a number of different applications, it has been specifically validated for use with our Z'-LYTE™ assay technology to identify novel kinase inhibitors.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 0.1mM EDTA, 0.1mM PMSF, 150mM NaCl, 10mM glutathione, 25% glycerol, 0.25mM DTT and no preservative; pH 7.5
• Molecular Weight (g/mol): 90.3-56.6-81.4 kDa
• Name: Human AMPK alpha-1/beta-2/gamma-3, GST Tag, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Common Name: AMPK alpha-1/beta-2/gamma-3
• KinaseFamily: CAMKL Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag, His-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Full Length, Recombinant, Active
• Form: Liquid