Invitrogen™ Amphiphysin Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA521483

Product Code. 13217498

Description

Recommended positive controls: mouse hippocampus, mouse brain, rat brain. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (91%), Pig (100%), Chicken (95%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrin-mediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.

Specifications

• Antigen: Amphiphysin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Amph
• Gene Alias: Amph; AMPH1; amphiphysin; amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen); amphiphysin 1; amphiphysin I; HGNC:471
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 218038, 273, 60668
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.9 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O08838, P49418, Q7TQF7
• Gene Symbols: Amph
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Amphiphysin. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG