AMBRA1 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA520512

Product Code. 12828692

Description

A suggested positive control is rat brain tissue lysate. PA5-20512 can be used with blocking peptide PEP-0632.

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.

Specifications

• Antigen: AMBRA1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: AMBRA1
• Gene Alias: 2310079H06Rik; A130023A14; AA474864; activating molecule in beclin-1-regulated autophagy; activating molecule in Beclin1-regulated autophagy; activating molecule in BECN1-regulated autophagy protein 1; AMBRA1; autophagy and beclin 1 regulator 1; autophagy/beclin 1 regulator 1; autophagy/beclin-1 regulator 1; AV021921; D030051N19Rik; DCAF3; DDB1 and CUL4 associated factor 3; hypothetical protein; hypothetical protein LOC517263; ischemia related factor NYW-1; Kiaa1736; mKIAA1736; Nyw1; WD repeat domain 94; WDR94
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 228361, 55626, 59319
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: A2AH22, Q9C0C7
• Gene Symbols: AMBRA1
• Immunogen: An 18 amino acid peptide from near the amino terminus of human Ambra1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG