UltraPure Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4. When mixtures are extracted with UltraPure Buffer- Saturated Phenol, proteins are denatured and collect in the organic phase or at the interphase, while most nucleic acids remain in the aqueous phase. UltraPure Buffer-Saturated Phenol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles.
Performance and Quality Testing: The appearance of the solution is evaluated at room temperature. No RNase or DNase activity detected.
DNA and RNA Purification and Analysis, DNA Extraction, General gDNA Purification Reagents and Accessories, Genomic DNA Purification, minutesiprep, Plasmid DNA Purification, RNA Extraction, Total RNA Isolation