Invitrogen™ Ambion™ ULTRAhyb™ Ultrasensitive Hybridization Buffer

Proprietary blend of hybridization accelerators and blocking agents that greatly enhance the levels of hybridization

Overview
Brand: Invitrogen™

Manufacturer Part Number: AM8670

125 ML ULTRAHYB, ULTRASENSITIVE HYBRIDIZATIONbuffer 125mL Store at 4°C

UNSPSC: 41105326

Code: 50

Additional Details:
Additional Details: Weight: 0.10500kg



Product Code. 10289154

Quantity Price
1 £ 142.0 / Each
EU Stock 17
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Buffer Hybridization
Quantity 125mL
Storage Requirements 4°C

  • Signals that once took days to visualize are now apparent in hours
  • It is supplied in four bottles containing 125mL each
  • Increase the sensitivity of blot hybridization experiments
  • Increase random-primed DNA probe sensitivity up to 100x; RNA probe sensitivity 20x
  • Decrease hybridization time to 2 hours
  • Using standard hybridization buffers, only 1 to 5% of target molecules on a blot hybridizes to probe, making blots a relatively insensitive method for nucleic acid analysis (Vernier et al. (1996) Anal Biochem 235: 11-19); With ULTRAhyb Ultrasensitive Hybridization Buffer, the hybridization reaction approaches completion, so that as few as 10,000 molecules can be detected
  • Compatible with RNA and DNA probes labeled isotopically and nonisotopically in northerns, Southerns, and dot/slot blots
  • Contains 50% formamide and is compatible with positively charged membranes
  • Requires 10-100x less nonisotopic DNA probe than standard hybridization buffers, making it possible to perform many more hybridizations with a single labeling reaction

Applications:

DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting

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