Ambion™ TRIzol™ Max Bacterial RNA Isolation Kit

Intact total RNA isolation from gram-positive and -negative bacteria

Overview
£292.50 valid until 31/12/2016
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Brand: Invitrogen

Manufacturer Part Number: 16096040

X2 TRIZOL MAX BACTERIAL RNA KIT

UNSPSC: 41105514

Code: 50

Additional Details:
Additional Details: Weight: 0.68000kg Transport: UN number : 1760 Chem class : 1b Pack group : II



Product Code. 11548766

Quantity Price
1 £ 410.0 / Pack of 2
Estimated Shipment date
from Supplier 13-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Final Product Type Total RNA, Transcriptome RNA, micro RNA
For Use With (Application) Cloning, Northern Blotting, Nuclease Protection Assays, RT-PCR (Endpoint), Real-Time PCR, cDNA Library Construction
Isolation Technology Organic Extraction
No. of Samples 200
Quantity 200 preps
Sample Size Up to 1 x 108 cells
Sample Type Bacteria (Gram-Negative), Bacteria (Gram-Positive)
Target Total RNA, Transcriptome RNA, miRNA
Test Time 1 hr
Yield 70μg

The TRIzol Max Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max Bacterial Enhancement Reagent and TRIzol Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.
Two Products in One Kit The TRIzol Max Bacterial RNA Isolation Kit combines Max Bacterial Enhancement Reagent with TRIzol Reagent. A 5-minute pre-treatment of bacteria with Max Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol Reagent generates a high-quality, minimally degraded RNA product.
Purified RNA is Ideal for Use with a Variety of Applications and Products Bacterial RNA isolated using the TRIzol Max Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.

  • Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
  • Uses two technologies for maximum RNA yield and purity
  • Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps
    • Not high-throughput compatible (Manual)

      DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Bacterial Samples

Documentation

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