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Invitrogen™ TRI Reagent™ Solution

Isolate DNA, RNA and protein with a single reagent

Brand:  Invitrogen™ AM9738

102.06 GBP valid until 2019-02-28
Use promo code "13474" to get your promotional price.



Code : 50

Additional Details : CAS Number : 108-95-2 Weight : 0.10000kg Transport : UN number : 1760 Chem class : 8 Pack group : II

Product Code. 11312940

  • £114.00 / 100mL


EU Stock 176
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Tri Reagent™ solution is a complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.
Using TRI Reagent solution TRI Reagent starting material can be either fresh or frozen tissue, or cell samples of almost any size—the protocol is easily scalable. This RNA isolation method is well-recognized and referenced in numerous publications, a testament to its reliability.
How it works TRI Reagent combines phenol and guanidine thiocyanate in a monophase solution to facilitate the immediate inhibition of RNase activity. Biological samples are homogenized or lysed in TRI Reagent; the subsequent addition of bromochloropropane or chloroform results in the separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase. The RNA can then be precipitated from the aqueous phase with the addition of isopropanol. The entire protocol can be completed in approximately 1 hour.
Applications The isolated RNA is suitable for any downstream application, including RT-PCR, amplification for array analysis, hybridization assays, or in vitro translation. PCR, Southern blotting, and cloning can be performed using DNA isolated with TRI Reagent. Isolated proteins can be used in SDS-PAGE and western blotting.
Tri Reagent solution is incorporated into our RiboPure and MagMAX-96 for Microarrays RNA isolation kits.

  • Simple: a single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein
  • Productive: obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods
  • Versatile: works with many sample sources, including human, plant, yeast, bacterial and viral samples
  • Flexible: easily scalable depending on starting material and desired yield

Not high-throughput compatible (Manual)

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues, Total RNA from Bacterial Samples, Total RNA from Blood, Total RNA from FFPE Samples, Total RNA from Plant Cells, Total RNA from Yeast

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

Genomic DNA, Protein, Total RNA
Organic Extraction
Bacteria, Blood, Buccal Samples, Cells, Fungi, Liquid Samples (e.g. Serum), Plant Samples, Tissue, Viral Samples, Yeast
1 hr
Cloning, In Vitro Translation, Microarray Analysis, Northern Blotting, Nuclease Protection Assays, PCR, Real-Time PCR, Southern Blotting
100mL
Genomic DNA, RNA & Protein, Total RNA
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Special Offers

Special Offers

  • Big discounts are available on a range of popular products including chemicals, lab essentials, equipment, consumables, life science and safety products.
    : 13474

    2019-02-28

    *Discount will apply to qualifying orders received by Fisher Scientific between 01/01/2019 and 28/02/2019. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

    Terms & Conditions

For Research Use Only. Not for use in diagnostic procedures.