Store at -20°C. Do not store in a frost-free freezer.
RNase Cocktail™ is mixture of two highly purified ribonucleases, RNase A (500U/mL) and RNase T1 (20,000U/mL) and is free of DNase and nicking activities. Digestion of RNA with RNase A alone leaves fragments of RNA which are large enough to be visible on agarose gels and precipitate in ethanol. RNase A cuts after C and U residues, and RNase T1 cuts after G residues. Consequently, mixture of both enzymes results in reduction in RNA fragment size over use of either alone.
Use RNase Cocktail for all situations, desirable to degrade RNA, i.e. plasmid minipreps and ribonuclease protection assays
RNase Cocktail is supplied in 50% glycerol for maximum convenience
Unit concentration: RNase A at 500U/mL and RNase T1 at 20,000U/mL
Quality Control: RNase Cocktail Enzyme Mix is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity; functionality is determined in ribonuclease protection assay
Unit Definitions: RNase A: One unit of RNase A is amount required to give increase in absorption at 286nm of 0.0146 absorbance units per minute in a 1mL volume
Unit assay conditions: 100mM Tris-acetate (pH 6.5), 1mM EDTA and 1mM cyclic 2', 3'-CMP
RNase T1: 100 Units of RNase T1 is amount of enzyme that yields an increase in absorption at 260nm of 0.01428 units per min. at room temperature using 60μg/mL yeast total RNA as a substrate
DNA & RNA Purification & Analysis, DNA Extraction, Maxiprep, Midiprep, Miniprep, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis & Blotting, Plasmid DNA Purification