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Invitrogen™ RNAqueous™ Total RNA Isolation Kit

The RNAqueous Kit is for phenol-free total RNA isolation from 0.1–0.5 g of tissue or from 107 to 108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

Brand:  Invitrogen™ AM1912

329.65 GBP valid until 2024-09-17
Use promo code "21690" to get your promotional price.



Code : 50

Additional Details : Weight : 0.53000kg Transport : UN number : 1760 Chem class : 8 Pack group : III

Product Code. 10075994

  • £382.00 / Each
In Stock
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

The RNAqueous Kit is for phenol-free total RNA isolation from 0.1–0.5 g of tissue or from 107 to 108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

  • Convenient syringe-based filtration
  • Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Not high-throughput compatible (Manual)

Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues

Specifications

Specifications

• 6 mL Formaldehyde Load Dye; store at -20°C
• 60 mL Lysis/Binding Solution; 4°C
• 60 mL Water; 4°C
• 4 mL Lithium Chloride Precipitation Solution; 4°C
• 40 mL Wash Solution #1; 4°C
• 80 mL Wash Solution #2/3 (concentrated); 4°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes; room temperature
• 10 mL Elution Solution; room temperature
50–120 μL
Total RNA
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Room Temperature
Spin Column (Glass Fiber Filter)
Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
Not High-throughput Compatible (Manual)
50 Preps
Bacteria: ≤109 cells
Cells: ≤107
Tissue: ≤75 mg
Plant: ≤75 mg
Yeast: ≤108 cells
Virus: varies
Enzymatic Reactions: varies
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For Research Use Only. Not for use in diagnostic procedures.