Invitrogen™ RNAqueous Total RNA Isolation Kit

Product Code. 10596935

Brand: Invitrogen™ AM1912M

Description

Uses glass fiber filter separation technology

The RNAqueous Kit is for phenol-free total RNA isolation from 0.1g to 0.5g of tissue or from 10⁷ to 10⁸ cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Not high-throughput compatible (Manual)

Applications:

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues

Specifications

• Isolation Technology: Spin Column (Glass Fiber Filter)
• Sample Type: Bacteria, Cells, Plant Samples, Tissue, Viral Samples, Yeast
• Final Product Type: Total RNA
• For Use With (Application): Northern Blotting, Nuclease Protection Assays, RT-PCR (Endpoint), Real-Time PCR, cDNA Library Construction
• Includes: 6mL Formaldehyde Load Dye; 60mL Lysis/Binding Solution; 60mL Water for 64% Ethanol; 4mL Lithium Chloride Precipitation Solution; 40mL Wash Solution 1; 80mL Wash Solution 2/3 Concentrate; 50 Filter Cartridges; 100 Collection Tubes; 10mL Elution Solution; Manual
• Product Type: Kit with manual
• Quantity: 50 Preps
• Target: Total RNA