Invitrogen™ RNAqueous™-96 Total RNA Isolation Kit

Product Code. 10137304

Brand: Invitrogen™ AM1920

Description

The RNAqueous™-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

The RNAqueous™-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains sufficient reagents for 192 (2 x 96) RNA purifications. The RNAqueous™-96 Kit is ideal for the isolation of total RNA from multiple samples and very small samples.

Using the RNAqueous™-96 Kit
The procedure uses an RNA-binding glass-fiber filter and either vacuum filtration or centrifugation methods to provide high yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. The 96-well plates are compatible with most common 96-well plate vacuum manifolds and 96-well plate centrifuge rotors. The RNAqueous™-96 Kit provides consistently high yields of RNA from various amounts of starting material. The high quality of RNA isolated is illustrated (see figure).

Accessory product
The RNAqueous™-96 Automated Kit (Cat. No. AM1812) is the same product in a format suitable for automation.

Specifications

• Content And Storage: • 50 mL Nuclease-free Water; store at room temperature
  • 50 mL Elution Solution; room temperature
  • 2 Filter Plates; room temperature
  • 2 Collection Plates; room temperature
  • 2 Adhesive Plate Sealers; room temperature
  • 2 Plate Lids; room temperature
  • 50 mL Lysis/Binding Solution; 4°C
  • 175 mL Wash Solution (concentrated); 4°C
  • 53 mL Rebinding Mix; 4°C
  • 12 mL DNase I Buffer; -20°C
  • 1.25 mL DNase I; -20°C
• Isolation Technology: Silica Plate
• Elution Volume: ≥50 μL
• Sample Type: Cells, Tissue
• Final Product Type: Total RNA
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Equipment): MultiPROBE™ II HT
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Quantity: 192 Preps
• Shipping Condition: Box 1: Dry Ice
  Box 2: Room Temperature
• Starting Material Amount: Cells: ≤10⁶
  Tissue: ≤1.5 mg

For Research Use Only. Not for use in diagnostic procedures.