Invitrogen™ Ambion™ Retic Lysate IVT Kit

Efficiently translates high molecular weight proteins and both capped and uncapped mRNAs

Overview
Brand: Invitrogen™

Manufacturer Part Number: AM1200M

60 RXN RETIC LYSATE IVT KIT WITH MANUAL 60REACTION

UNSPSC: 41116134

Code: 50

Additional Details:
Additional Details: Weight: 0.04000kg



Product Code. 10005314

Quantity Price
1 £ 185.0 / Each
EU Stock 4
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) Cell Free Protein Expression for Functional Analysis, Cell-Free (in vitro) Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Includes Retic Lysate, 20X Translation Mix minus-Met, 20X Translation Mix minus-Leu, High Salt Mix minus-Met, Low Salt Mix minus-Met, Capped Xef-1 RNA, and Nuclease-Free water
Product Type Kit with manual
Quantity 60 reactions
Storage Requirements Retic Lysate should be stored at -80°C. 20X Translation Mix minus-Met, 20X Translation Mix minus-Leu, High Salt Mix minus-Met, Low Salt Mix minus-Met, and Capped Xef-1 RNA should be stored below -70°C. Nuclease-Free water can be stored at any temperature.

The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs. The optimized lysate results in high protein yield, biological activity, and consistent performance. A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon. The Retic Lysate IVT Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG.

  • Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs
  • Accommodates large volume of template mRNA
How it works
The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA. Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers. These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences.