Invitrogen™ QuantumRNA™ β-Actin Internal Standards

Used for quantitative end-point RT-PCR

Brand:  Invitrogen™ AM1720

Product Code. 10744227

Description

The Invitrogen™ QuantumRNA™ β-actin Internal Standards with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal β-actin primers that cannot be extended. By adjusting the ratio of β-actin Competimer™s to normal β-actin mRNA primers, the signal for β-actin mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. β-actin mRNA is an abundantly expressed &ldquohousekeeping” gene; therefore, it is difficult to multiplex primers for β-actin mRNA in the same PCR reaction with primers for rare messages. Typically the β-actin mRNA signal will overwhelm the relative RT-PCR reaction, and the rare gene-specific product goes undetected. β-actin Standards Product Size: 294 bp

Specifications

• Content And Storage: β-Actin Primer Pair and β-Actin Competimer™s are stored at –20°C. Control Template RNA should be stored below –70°C. Nuclease-free water may be stored at any temperature.
• Format: Tube(s)
• Label or Dye: Unlabeled
• Shipping Condition: Dry Ice
• Target: β-actin
• Size (Final Product): 294 bp

• Control Template: Total RNA from Mouse Liver
• Gene Symbol: ACTB
• Product Line: Ambion™, QuantumRNA™
• Species: Mouse
• No. of Reactions: 100 Reactions