Invitrogen™ PureLink™ RNA Mini Kit

Provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol

Brand:  Invitrogen™ 12183018A

Product Code. 10307963

Description

Rapid and convenient column purification
The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000μg of purified RNA from a single extraction. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.

Simplified, nontoxic RNA purification
The PureLink™ RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.

• Obtain high-yield, high-integrity RNA
• Rapid, non-hazardous RNA purification capabilities
• Ready-to-use RNA for many downstream applications

Specifications

• Content And Storage: • 125 mL Lysis Buffer; store at room temperature
  • 50 mL Wash Buffer I; room temperature
  • 15 mL Wash Buffer II; room temperature
  • 15.5 mL RNase-free Water; room temperature
  • 50 PureLink Spin Cartridges with Collection Tubes; room temperature
  • 50 Collection Tubes; room temperature
  • 50 Recovery Tubes; room temperature
• Elution Volume: 30–300 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Scale: Mini
• Starting Material Amount: Bacteria: ≤10⁹ cells
  Blood: ≤0.2 mL
  Cells: ≤5 x 10⁷
  Plant: ≤250 mg
  Tissue: ≤200 mg
  Yeast: ≤5 x 10⁸ cells
  Enzymatic reactions: ≤1.2 mL

• Isolation Technology: Silica Spin Column
• Sample Type: Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions (DNase digestions, RNA labeling/cleanup preps, IVT)
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Yield: Up to 350 μg

Safety and Handling

Product Identifier

• PURELINK RNA MINI KIT

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 1 C
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : DyLight Reactive Dyes, Thiocyanic acid