Invitrogen™ Ambion™ mMESSAGE mMACHINE™ SP6 Transcription Kit

in vitro synthesis of large amounts of capped RNA

Overview
Brand: Invitrogen™

Manufacturer Part Number: AM1340

25 RXN MMESSAGE MMACHINE, SP6 KIT 25REACTIONSStore at -20 C store at - 20°C

UNSPSC: 41105804

Code: 50

Additional Details:
Additional Details: Weight: 0.07000kg



Product Code. 10391175

Quantity Price
1 £ 391.0 / Each
EU Stock 57
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) Gene Expression Analysis and Genotyping, in vitro Transcription
Includes SP6 Enzyme Mix, 10X Reaction Buffer, 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, Gel Loading Buffer II, and Nuclease-free Water
Product Type Kit
Quantity 25 reactions

mMESSAGE mMACHINE™ kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end.

  • RNA Yield: 5-25μg
  • Include cap analog [m7G(5')ppp(5')G] in an ultra high-yield transcription reaction
  • Cap analog is incorporated only as first or 5' terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
  • Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
  • Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications

Documentation

Technical documents