Invitrogen™ Ambion™ MEGAshortscript™ T7 Transcription Kit

Maximize the yield of short RNA transcripts

Overview
Brand: Invitrogen™

Manufacturer Part Number: AM1354M

MEGASHORTSCRIPT T7 KITreactions

UNSPSC: 41105804

Code: 50

Additional Details:
Additional Details: Weight: 0.07000kg



Product Code. 10575975

Quantity Price
1 £ 287.0 / 30 reactions
Estimated Shipment date
from Supplier 14-12-2016
Add to basket

For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) Gene Expression Analysis and Genotyping, in vitro Transcription
Includes T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-RNA 18S, TURBO DNase, Ammonium Acetate Stop Solution, Gel Loading Buffer II, and Nuclease-free Water
Product Type Kit with manual
Quantity 25 reactions
Storage Requirements T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-RNA 18S, TURBO DNase, Ammonium Acetate Stop Solution, and Gel Loading Buffer II are all stored at -20°C. Nuclease-free Water may be stored at any temperature.

The MEGAshortscript T7 Kit is designed for high yields of in vitro-transcribed RNA products, in the 20 to 500 nucleotide range. Using conventional methods, transcription of small templates often results in low mass yields of RNA. Short transcripts require many more transcription initiation events to synthesize a given mass of product. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript T7 Kit has been designed to overcome the drawbacks involved in conventional in vitro transcription to maximize the yield of short RNA transcripts.

  • Efficiently transcribes small templates (<300 bases) with consistently high yields
  • Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates