Invitrogen™ Linear Acrylamide (5 mg/ml) (1 ml Tube)

Product Code. 10638105

Brand: Invitrogen™ AM9520

Description

Invitrogen Linear Acrylamide (5 mg/mL) is for use as a nucleic acid coprecipitant and is ideal for PCR and RT-PCR reactions since small amounts of contaminating nucleic acids present in other carriers could be amplified.

Invitrogen Linear Acrylamide (5 mg/mL) is for use as a nucleic acid coprecipitant and is ideal for PCR and RT-PCR reactions since small amounts of contaminating nucleic acids present in other carriers could be amplified.

• Ideal for RT-PCR
• Increases pellet mass
• Quantitative recovery of low concentrations (ng/mL) of nucleic acid
• Prevents pellet loss in nuclease protection assays

What is a Coprecipitant?
Co-precipitants are inert substances used to aid recovery of nucleic acids during alcohol precipitations. While they can be used for precipitating large amounts of nucleic acids, they are essential for quantitative recovery of small amounts of nucleic acids in dilute solutions. Often, the use of such molecules is desirable for no other reason but visualization of the pelleted precipitate after centrifugation. Linear acrylamide offers advantages over other coprecipitants in that it is chemically synthesized and not treated with reagents (e.g., proteases) derived from biological sources. Therefore, it is not contaminated with biological material making it ideal for use upstream of RT-PCR. It has been shown to precipitate pictogram amounts of DNA fragments larger than 20 base pairs while failing to precipitate shorter fragments and free nucleotides. Linear acrylamide is useful for separating reaction products from unincorporated nucleotides and from most oligonucleotide primers. It should be used at a final working concentration of 10 to 20 μg/mL. Linear acrylamide will not interfere with A_260/280 readings. Supplied in 5 x 1 mL tubes.

Order Info

Shipping Condition: Dry Ice

Specifications

• Chemical Name or Material: Acrylamide
• Content And Storage: Store at –20°C.
• Concentration: 5 mg/mL
• For Use With (Application): RNA Extraction
• Product Line: Ambion™
• Purity: Molecular Biology Grade
• Quantity: 5 x 1mL tubes
• Shipping Condition: Dry Ice
• Form: Liquid

Safety and Handling

Product Identifier

• Linear Acrylamide

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 1B
• Germ cell mutagenicity Category 1B
• Reproductive toxicity Category 2
• Skin sensitiser Category 1

Hazard Statement

• H317-May cause an allergic skin reaction.
• H340-May cause genetic defects.
• H350-May cause cancer.
• H361f-Suspected of damaging fertility.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Acrylamide

For Research Use Only. Not for use in diagnostic procedures.