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Invitrogen™ Illumina™ TotalPrep™ RNA Amplification Kit
Description
The new TotalPrep RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous Illumina RNA Amplification Kit.
New improvements for a tested and reliable amplification procedure
The TotalPrep RNA Amplification Kit is based on the RNA amplification protocol developed in the laboratory of James Eberwine. The procedure consists of reverse transcription with an oligo(dT) primer bearing a T7 promoter (TTTT-PT7) using ArrayScript RT, a proprietary reverse transcriptase (RT) engineered to produce higher yields of first-strand cDNA than wild type enzymes. ArrayScript RT catalyzes the synthesis of virtually all full-length cDNA, which is the best way to ensure production of reproducible microarray samples. The cDNA then undergoes second-strand synthesis and clean-up to become a template for in vitro transcription with T7 RNA Polymerase. To maximize cRNA yield, the proprietary MEGAscript™ in vitro transcription (IVT) technology, along with biotin UTP (provided in the kit), is used to generate hundreds to thousands of biotinylated, antisense RNA copies of each mRNA in a sample.
- Improved first-strand cDNA synthesis using ArrayScript™ reverse transcriptase
- Biotinylated UTP allows ease of use and optimal labeling
- Maximization of cRNA yield using MEGAscript™ IVT technology
- Significantly improved sensitivity starting with just 50ng of input RNA
Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, RNA Amplification
Order Info
Shipping Condition: Approved for shipment on Wet or Dry Ice
Specifications
Specifications
| Product Type | RNA Amplification Kit |
| Content And Storage | The following components should be stored at -20°C in a non-frost-free freezer: • 26 μl T7 Oligo(dT) Primer • 51 μl 10X First Strand Buffer • 205 μl dNTP Mix • 26 μl RNase Inhibitor • 27 ArrayScript™ Reverse Transcriptase • 252 μl 10X Second Strand Buffer • 51 μl DNA Polymerase • 26 μl RNase H • 64 μl T7 10X Reaction Buffer • 64 μl T7 Enzyme Mix • 1.75 ml Nuclease-free Water (may be stored at any temperature) • 10 μl 1 mg/ml Control RNA Store the following at room temperature: • 10 ml Nuclease-free Water (may be stored at any temperature) • 30 ml Wash Buffer (may be stored at 4°C) • 7 ml cDNA Binding Buffer • 9 ml cRNA Binding Buffer • 24 cRNA Filter Cartridges • 48 cRNA Collection Tubes • 30 cDNA Filter Cartridges + Tubes • 30 cDNA Elution Tubes |
| Includes Label or Dye | Yes |
| Labeling Method | Direct Labeling |
| Reverse Transcriptase | ArrayScript™ |
| Sample Type | Poly(A+) RNA, Total RNA |
| System Type | Illumina Sentrix™ |
| Format | Kit |
| Product Line | Ambion, TotalPrep |
| Quantity | 24 Reactions |
For Research Use Only. Not for use in diagnostic procedures.
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