Ambion™ Power SYBR™ Green Cells-to-CT™ Kit

Room temperature sample preparation in only 7 minutes

Overview
£1676.25 valid until 31/12/2016
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Brand: Invitrogen

Manufacturer Part Number: 4402955

400 RXN SYBR, GREEN CELLS-TO-CT KIT 400REACTIONSStore at -20 C

UNSPSC: 41106302

Code: 50

Additional Details:
Additional Details: Weight: 0.34000kg



Product Code. 10259494

Quantity Price
1 £ 2305.0 / Each
Estimated Shipment date
from Supplier 07-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Detection Method SYBR
Dye Type SYBR™ Green
For Use With (Equipment) 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System, GeneAmp™ 5700, GeneAmp™ 9600, StepOnePlus™, Standard Mode
Quantity 400 reactions
Sample Type Cells

Ambion Power SYBRGreen Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results. A breakthrough cell lysis and RNA stabilization technology eliminates the laborious and time-consuming RNA purification process completely. Power SYBRGreen Cells-to-CT Kit is Powered by AmpliTaq Gold™ DNA Polymerase, LD, which provides the highest levels of specificity with standard real-time PCR.

  • Includes optimized reagents to work efficiently, right out of the box
  • Simple, effective Cells-to-CT technology enables sample preparation at room temperature in only 7 minutes, including DNase treatment
  • Validated accuracy, exceptional reproducibility, and maximal sensitivity from 10 to 100,000 cells per sample; results equivalent to those from purified RNA
Extraordinary Value The kit includes reagents for sample preparation integrated into a complete, optimized gene expression workflow including reverse transcription reagents and a high-performance PCR master mix with SYBRGreen.
Extraordinary Ease The protocol begins with a simple 7-minute sample preparation procedure. First, cells are washed in PBS, and then lysed for 5 minutes at room temperature; DNase treatment can be performed concurrently. The lysis reaction is stopped, and the sample is then ready for reverse transcription or storage at -20°C for up to 5 months. Because samples are processed directly in culture plates (96- or 384-well), sample handling and the potential for sample loss or transfer error are minimized, resulting in higher reproducibility. Following sample preparation, a portion of the cell lysate is added to an RT reaction followed by real-time PCR using the included master mix.
Extraordinary Performance To demonstrate the performance of the kit, replicate experiments were run using either traditional RNA purification and real-time PCR analysis, or the Power SYBRGreen Cells-to-CT Kit. The kit shows equivalent performance to results obtained using purified RNA for analysis of 10 to 100,000 cells. In addition, the sensitivity, efficiency, and dynamic range were shown to be superior to competitor lysate kits. Samples prepared with the kit have been tested for compatibility on various real-time PCR platforms including those from Applied Biosystems, Roche, and Bio-Rad.

  • PCR Method: 2-Step qRT-PCR
  • Purity/Quality Grade: LD (Low DAN)
  • Passive Reference Dye: ROX (Pre-mixed)
  • Polymerase: AmpliTaq Gold™ DNA Polymerase

Accessory Product:
The SYBRGreen Cells-to-CT Control Kit (Cat. No. 4402959) contains a Xeno™ RNA control and primer set and is recommended to monitor the efficiency of cell lysis and amplification inhibition in Cells-to-CT reactions.

Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real-Time PCR Directly from Samples

Documentation

Technical documents

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