Invitrogen™ Ambion™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility

Brand: Invitrogen™

Manufacturer Part Number: AM9516

GLYCO BLUE COPRECIP 5 X 300ULAmbion® GlycoBlue consists of a blue dye

UNSPSC: 41105514

Code: 50

Additional Details:
Additional Details: Weight: 0.50000kg

Product Code. 10301575

Quantity Price
1 £ 154.0 / 1500µL
EU Stock 31
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Quantity 5 x 300μL
Storage Requirements Store at -20°C.

GlycoBlue Coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate, which is useful as a nucleic acid coprecipitant. The attached dye increases visibility of the pellet. This product is an ideal coprecipitant in nuclease protection assays at 1/100 dilution of stock solution.

  • Ideal for RT-PCR
  • Increases pellet mass and visibility
  • Quantitative recovery of low concentrations (ng/mL) of nucleic acid
  • Prevents pellet loss in nuclease protection assays

What is a coprecipitant?
Co-precipitants are inert substances used to aid recovery of nucleic acids during alcohol precipitations. While they can be used for precipitating large amounts of nucleic acids, they are essential for quantitative recovery of small amounts of nucleic acids in dilute solutions. Often, the use of such molecules is desirable for no other reason but visualization of the pelleted precipitate after centrifugation. GlycoBlue Coprecipitant can be added to nucleic acid solutions at a final concentration of 50 to 150μg/mL. When a typical acetate/alcohol precipitation is done, the GlycoBlue Coprecipitant will precipitate with the nucleic acids, facilitating good RNA or DNA recovery while increasing the size and visibility of the pellet. Since glycogen does not contain appreciable amounts of nucleic acids, it is often preferable to yeast RNA as a coprecipitant, especially in applications where nucleic acid mass is being assessed or where added nucleic acid could interfere or compete with subsequent enzymatic reactions. The glycogen used is isolated from mussel, a biological source, as are most other preparations of this coprecipitant. Glycogen is treated with Proteinase K and SDS to remove any contaminating nucleases, and then phenol/chloroform extracted, ethanol precipitated, and resuspended in nuclease-free water. The glycogen is guaranteed RNase- and DNase-free.

DNA and RNA Purification and Analysis, DNA Extraction, General RNA Purification Reagents and Accessories, General gDNA Purification Reagents and Accessories, Genomic DNA Purification, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues