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Invitrogen™ FirstChoice™ RLM-RACE Kit

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

Brand:  Invitrogen™ AM1700M

Code : 50

Additional Details : Weight : 0.00450kg

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Product Code. 10066184

  • £574.00 / Each

In stock, ready to ship: 6

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For Research Use Only. All usage must comply with product instructions.
 

Requires : SuperTaq Thermostable Taq DNA Polymerase sold separately. For optimal amplification of fragments ≥1 kb, use SuperTaq-Plus.

Description

Description

The FirstChoice RLM-RACE Kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages.

  • From RNA to PCR product in less than a day
  • Yields single, specific product from rare transcripts
  • Selects 5' and/or 3' ends of true messages
  • Efficient—
  • Al enzymatic reactions are optimized to ensure detection of even rarest mRNA
  • Final product size: 7kb or less
  • Optimal reaction temperature: 42°C
  • Sample: RNA

  • Rapid amplification of cDNA ends (5'-RACE) is polymerase chain reaction-based technique developed to facilitate cloning of sequence from the 5'-ends of messages
  • Improvement to basic RACE protocol
  • Selects full-length mRNA—no rRNA, tRNA or degraded mRNA

Optimal reaction temperature: 42°C

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

Specifications

Specifications

cDNA
Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate™ GAPDH-M, and 0.5 M EDTA should be stored at –20°C. Nuclease-free water may be stored at any temperature.
Separate Components
Ambion™, FirstChoice®
Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA should be stored at -20°C. Nuclease-free water may be stored at any temperature and manual
42° C
M-MLV
Videos
Documents

For Research Use Only. Not for use in diagnostic procedures.