Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Brand:  Invitrogen™ 11753100

Product Code. 10043982

Description

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

• Efficiency: offers significant time and cost savings for increased throughput
• Convenience: enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
• Sensitivity: no separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad detection range: detect targets from samples ranging from a single cell to 10,000 cells
• Versatility: compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)

Streamline your workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for your qRT-PCR application
CellsDirect technology is validated for gene expression studies involving limited samples. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™ or LUX™ fluorogenic primers).

This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit with ROX Reference Dye included in the 2X Reaction Mix is also available.

Specifications

• Content And Storage: • 10 mL Resuspension Buffer
  • 1 mL Lysis Enhancer
  • 500 μL DNase I, Amplification Grade (1 U/μL)
  • 160 μL 10X DNase I Buffer
  • 400 μL 25 mM EDTA
  • 100 μL SuperScript™ III RT/Platinum™ Taq Mix
  • 2 × 1.25 mL 2X Reaction Mix
  • 1 mL 50 mM MgSO₄
  • 2 mL DEPC-treated water
  • 10 μL HeLa Total RNA (10 ng/μL)
  • 100 μL ROX™ Reference Dye (store at -20°C in the dark)
  The kit is shipped on dry ice and should be stored at -20°C
• Reaction Speed: Fast or Standard
• Product Line: CellsDirect™, Platinum™, SuperScript™
• Concentration: 2X
• Sample Type: Cells

• Detection Method: Primer-probe
• For Use With (Equipment): 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
• For Use With (Application): RT-PCR
• No. of Reactions: 100 reactions

For Research Use Only. Not for use in diagnostic procedures.