Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ±0.2. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, and easier alternative to preparing solutions from crystalline phenol.
DNA and RNA Purification and Analysis, General RNA Purification Reagents and Accessories, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues