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alpha GalCer:CD1d Complex, PE, clone: L363, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-2019-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15248719

  • £180.65 / 100µg


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Description

Description

Description: This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes.Applications Reported: This L363 antibody has been reported for use in flow cytometric analysis.Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylceramide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

alpha GalCer:CD1d complex
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P11609
alpha GalCer-CD1d complex, alphaGalCerCD1d complex, alpha galcer-CD1d complex, alpha-galactosylceramide, alpha galactosylceramide, CD1d
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
L363
PE
Cd1d1
Conjugated
Cd1d1
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12479
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