alpha GalCer:CD1d Complex Monoclonal Antibody (L363), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
17111192	Affymetrix eBioscience 16-2019-025	25 mg
17121192	Affymetrix eBioscience 16-2019-050	50 mg

Description

This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation. The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes. Applications Reported: This L363 antibody has been reported for use in functional assays. Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylcermide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

alpha GalCer:CD1d Complex Monoclonal antibody specifically detects alpha GalCer:CD1d Complex in Mouse samples. It is validated for Flow Cytometry, Functional Assay

Specifications

• Antigen: alpha GalCer:CD1d Complex
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Alias: AI747460; Antigen-presenting glycoprotein CD1d1; Cd1.1; Cd1a; Cd1d; CD1d.1; Cd1d1; CD1d1 antigen; Ly-38; T-cell surface glycoprotein CD1d1
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: L363
• Applications: Flow Cytometry, Functional Assay
• Conjugate: Functional Grade
• Host Species: Mouse
• Target Species: Mouse
• Gene Accession No.: P11609, Q9JHE4
• Gene ID (Entrez): 12479, 53897
• Primary or Secondary: Primary
• Content And Storage: 4° C