TCR alpha/beta, FITC, clone: WT31, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 11-9955-42
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: This WT31 monoclonal antibody recognizes the human alpha beta T cell receptor (TCR) complexed with the CD3 epsilon chain. Composed of a 44-kDa alpha and a 37-kDa beta chain, TCR specificity is typically determined by V alpha, J alpha, V beta, D beta, and J beta gene rearrangement. The TCR is often associated with the CD3 complex, which is composed of at least five polypeptides. The TCR/CD3 complex recognizes and binds to major histocompatibility complex-bound antigen presented by antigen presenting cells such as B cells, dendritic cells, and macrophages. These events lead to T cell activation, resulting in cellular signaling and cytokine production.The WT31 monoclonal antibody has been reported to induce proliferation of resting T cells, as well as inhibit cytotoxic T cell activity. In addition, some antibodies against CD3 partially inhibit alpha beta TCR antibody staining using WT31; clone SK7 (anti-human CD3) is recommended for use in two-color experiments.Applications Reported: This WT31 antibody has been reported for use in flow cytometric analysis.Applications Tested: This WT31 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|alpha beta TCR|
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Tcrb, Tcra, FLJ22602, MGC117436, MGC22624, MGC23964, MGC71411, TCRA, TCRD, TRA, T-cell antigen receptor, alpha polypeptide, T-cell receptor, TCR beta|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.