Invitrogen™ alpha Actinin 2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA527863

Product Code. 13421827

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, GL261, C8D30, NIH-3T3, Rat heart. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (94%), Pig (99%), Chicken (98%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

Specifications

• Antigen: alpha Actinin 2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Actn2
• Gene Alias: 1110008F24Rik; actinin alpha 2; actinin, alpha 2; ACTN2; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; CMD1AA; CMH23; F-actin cross-linking protein
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100157406, 11472, 291245, 88
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.18 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P35609, Q9JI91
• Gene Symbols: Actn2
• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Pig, Rat
• Product Type: Antibody
• Isotype: IgG