Invitrogen™ Human ALK [F1174L], GST Tag, His Tag Recombinant Protein

Product Code. 15418833

Brand: Invitrogen™ PV6161

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Human ALK F1174L, internal fragment, amino acids L1066-S1437 (as in NCBI/Protein entry NP_004295.2), F1174L mutation, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Related Product: LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant

The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50 mM HEPES with 100 mM NaCl, 15 mM glutathione, 20% glycerol, 5 mM DTT and no preservative; pH 7.5
• Molecular Weight (g/mol): 70.4 kDa
• Name: Human ALK [F1174L], GST Tag, His Tag
• Quantity: 100 μg
• Common Name: ALK (F1174L Mutant)
• Protein Length: 1066-1437
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag, His-tag
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Insect cells
• Form: Liquid