Invitrogen™ Alexa Fluor™ 488 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG

Product Code.	Quantity	unitSize
15631902	50 Slides	Pack of 50
15661892	Sufficient for 150 Slides	Pack of 150

Product Code. 15631902 Supplier Invitrogen™ Supplier No. B40943

Description

Features poly-HRP-conjugated goat anti-rabbit IgG secondary antibody. Alexa Fluor™ 488 Tyramide SuperBoost™ Kit combine brightness of AlexaFluor dyes with high signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce s

SuperBoost™ tyramide signal amplification is most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Features:

• High sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images, co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

Benefits:

• Enhancement of signal using Alexa Fluor tyramides: Utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules
• Poly-HRP enhancement: Several HRP enzymes are conjugated with short polymers, enhancing signal by several fold over regular HRP systems
• Reaction stop solution: Include an HRP stop solution to halt the HRP reaction
• Reduction of background: Include blockers for elimination or reduction of endogenous peroxidase and fluorescent background signals

Shelf Life:

• Stable for 6 months after receiving

Specifications

• Content And Storage: 1 kit sufficient for 50 microscope slides (18 x 18 mm), containing:
  
  • Blocking buffer (1X), 22.5 mL
  • Poly-HRP-conjugated goat anti-rabbit secondary antibody (1X), 22.5 mL
  • Alexa Fluor tyramide reagent
  • Hydrogen peroxide (stabilized 3% solution), 28.5 mL
  • Reaction buffer (20X), 6 mL
  • Reaction stop solution, 8 mg
  • Dimethylsulfoxide (DMSO), 200 μL
  
  Store at 2-8°C. When stored as directed, the product is stable for 6 months after receiving.
• Quantity: 50 Slides
• Shipping Condition: Approved for shipment at room temperature or on wet ice
• Conjugate: Alexa Fluor 488

Frequently Asked Questions (FAQs)

With a SuperBoost tyramide kit, I got excessive and non-specific labeling. What can I do to limit background and acquire a more localized labeling?

To limit background, we recommend performing a pre-blocking step with 3% H2O2 for 60 mins to inactivate endogenous peroxidases. To limit the localization of labeling, we recommend optimizing the final concentration of the primary and secondary antibodies and the dye-tyramide. You may also limit the incubation time of the dye-tyramide on the sample.

Is it possible to perform dual TSA labeling with SuperBoost tyramide kits?

Yes. This involves the sequential application of the antibodies and the tyramides with a HRP-quenching step between antibodies using H2O2.

How are SuperBoost tyramide kits different from the original TSA labeling kits?

The SuperBoost tyramide kits utilize poly-HRP labeled antibodies. This provides a greater number of horseradish peroxidase (HRP) molecules per antibody. The original kits used antibodies and streptavidin that were directly conjugated with HRP and thus, limited the number per antibody or streptavidin.

For Research Use Only. Not for use in diagnostic procedures.