Thermo Scientific™ Albumin, Bovine, Fraction V, 98%, Reagent Grade, pH 7.0

Albumin bovine/fraction V, CAS # 9048-46-8, also known as bovine serum albumin (BSA), is a serum albumin protein derived from cows.

Description

Albumin is used for enzyme-linked immunosorbent assay (ELISAs), immunoblots and immunohistochemistry. It acts as a blocking reagent particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. It is also used as a nutrient in cell and microbial culture. Further, it is used as a protein standard, diluent, enzyme stabilizer and in cell culture applications especially serum-free media.

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

General Description

• Albumin bovine/fraction V is a serum albumin protein commonly used in laboratory experiments including, enzyme-linked immunosorbent assay (ELISAs), immunoblot, and immune-histochemistry or -fluorescence

Applications

• Albumin bovine/fraction V displays blocking activity mainly when casein-sensitive antibodies, including phospho-specific antibodies, are used
• In genetic approaches this compound can be used to increase polymerase chain reactions (PCR) yields from low purity templates
• This compound is useful for stabilizing enzymes during storage and also for enzymatic reactions where the absence of nucleases is crucial
• In in vitro cultures, albumin bovine/fraction V has been applied as a nutrient in cell and microbial cultures especially when serum-free media is used
• It is commonly used as a protein standard to obtain calibration curves for several laboratory experiments
• Albumin bovine/fraction V is a small protein commonly used in immunohistochemistry to block nonspecific regions. The binding of this compound to nonspecific binding sites increases the probability of the antibodies bind only to the region of interest.

RUO – Research Use Only