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Molecular Probes™ alamarBlue™ Cell Viability Reagent

AlamarBlue™ Cell Viability Reagent

Brand:  Molecular Probes™ DAL1025

183.12 GBP valid until 2021-10-30
Use promo code "14806" to get your promotional price.



Code : 50

Additional Details : Weight : 0.10872kg

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Product Code. 10161053

  • £193.00 / Each

In stock, ready to ship: 17

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

alamarBlue reagent is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies.

Applications:

Cell proliferation determinations—alamarBlue reagent is water soluble, stable in culture, and non-toxic
Cell viability assays, including cytotoxicity—metabolic activity and dye generation changes in proportion to altered viability and cell damage
Cytokine assays—measure cytokine-induced proliferation, recover and expand cells at the end of the study if desired

alamarBlue reagent as a cell viability assay reagent
The alamarBlue assay is based on the ability of metabolically active cells to convert the reagent into a fluorescent and colorimetric indicator. Damaged and non-viable cells have lower innate metabolic activity, and generate a proportionally lower signal. The dye itself has low toxicity, and is generally safe for cells.

A safe, convenient, and versatile assay
alamarBlue reagent is an indigo-colored liquid that has been shown to effectively measure innate metabolic activity in animal, fungal and bacterial cells. Since alamarBlue reagent is non-toxic, the effector cells may be recovered for further analysis or cell expansion at the end of a study. alamarBlue reagent is water-soluble, saving time otherwise spend on washing and extraction steps. The homogeneous assay format of this kit is fast—add the single reagent directly to cells in suspension or to attached cells in full medium. The protocol is readily amenable to automation and high-throughput assays. The ability to detect the alamarBlue indicator by either fluorescence or spectroscopy provides compatibility with flow cytometry, plate readers and microscopy.

Experimental detection of alamarBlue reagent

Fluorometry: by excitation at 530–560 nm and emission at 590 nm
Spectrophotometry: by absorbance measurements at 570 nm and 600 nm
Specifications

Specifications

Bacteria, Eukaryotic Cells, Fungal Cells
Colorimetric, Fluorescent
530-560⁄590
1 to 4 hrs (up to 24 hrs for low cell numbers)
25mL
High-throughput Compatible
Blue
Resazurin
96-well plate, Cuvettes, 384-well plate
alamarBlue™
Wet Ice
Spectrophotometer, Microplate Reader
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For Research Use Only. Not for use in diagnostic procedures.