Eurofins DiscoverX Akt2/PKBβ (δPH, S474D) Protein, Active

Product Code. 17330154

Brand: Eurofins DiscoverX 14339M

Description

Active, recombinant human PKBβ, residues 120-481, S474D, containing an N-terminal His6 tag; activated by PDK1, for use in Kinase Assays.

Akt2, also known as Protein Kinase B Beta, is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. Akt2 serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.

EMBL M95936. The recombinant protein contains an amino acid substitution, S474D, with reference to the native sequence. This mimics the phosphorylation of S474.

Specifications

• Formulation: mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 1 mM EGTA, 1 mM benzamidine, 0.2 mM PMSF, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35. Frozen solution.
• Molecular Weight (g/mol): 42.8 kDa
• Quantity: 250 μg
• Species: Human
• Recombinant: Recombinant
• Protein Tag: N-terminal 6His
• Expression System: Recombinant enzyme expressed in Sf21 insect cells
• Protein Form: Truncated
• Purity or Quality Grade: ≥65%
• Protein: PKB beta (S474D)