AIRE Rat anti-Mouse, Alexa Fluor 488, Clone: 5H12, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-5934-82

Product Code. 15342050

Disclaimers:

For Research Use Only.

Description

This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE). This 58kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). However, expression of AIRE protein in the periphery remains unclear despite the detection of AIRE mRNA in the lymph node, lymphoid stromal cells, monocytes, and dendritic cells. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation.

The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2.

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Specifications

• Antigen: AIRE
• Clone: 5H12
• Conjugate: Alexa Fluor 488
• Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Host Species: Rat
• Quantity: 100μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Immunohistochemistry (Frozen)
• Concentration: 0.5mg/mL
• Format: Conjugated
• Gene Alias: Autoimmune Regulator
• Isotype: IgG2c
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine