AIRE, Alexa Fluor 488, clone: 5H12, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-5934-82

Product Code. 15342050

Description

Description: This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE). This 58-kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). However, expression of AIRE protein in the periphery remains unclear despite the detection of AIRE mRNA in the lymph node, lymphoid stromal cells, monocytes, and dendritic cells. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation.The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2.Applications Reported: This 5H12 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections (IHC-F).Applications Tested: This 5H12 antibody has been tested by immunohistochemistry on fixed, frozen mouse thymus at less than or equal to 10 ug/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: AIRE
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9Z0E3
• Gene Alias: AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy), autoimmune regulator, AIRE1, OTTHUMP00000109529, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Immunohistochemistry (Frozen)
• Clone: 5H12
• Conjugate: Alexa Fluor 488
• Gene: Aire
• Format: Conjugated
• Gene Symbols: Aire
• Isotype: IgG2c, kappa
• Quantity: 100μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 11634

For Research Use Only.