AIRE Monoclonal Antibody (TM-724), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15597596	Invitrogen 14-9534-80	25 μg
15507606	Invitrogen 14-9534-82	100 μg

Description

Description: This TM-724 monoclonal antibody reacts with human Autoimmune Regulator (AIRE). This 58 kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). AIRE mRNA in the periphery has been observed in the lymph node, fetal liver, and intestines. Furthermore expression has been observed in dendritic cells, epithelial cells and PBMCs. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation. As a result of alternative splicing, three isoforms have been reported. The conditions autoimmune-polyendocrinopathy-candiasis-ectodermal-dystrophy (APECED) and autoimmune polyglandular syndrome type 1 (APS-1) arise from mutations in the human AIRE gene. The TM-724 antibody is predicted to recognize all three isoforms of human AIRE (AIRE-1, AIRE-2, AIRE-3). Applications Reported: This TM-724 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This TM-724 antibody has been tested by immunohistochemistry on FFPE human thymus at less than or equal to 5 μg/mL. Low pH antigen retrieval is optimal although high pH antigen retrieval can also be used.

AIRE is a transcriptional regulator protein that binds to DNA as a dimer and tetramer, but not as a monomer, and may be involved in the regulation of gene expression. A nuclear protein, AIRE is a 545 amino acid protein with an HSR domain, two PHD type zinc fingers, and a SAND domain, and may be present in soluble high-molecular-weight complexes. It may form homodimer, homotetramer and also nuclear bodies and interacts with the transcriptional coactivator CBP and CREBBP. Three isoforms of AIRE are reported and one of them is predicted to be a candidate for nuclear-mediated decay (NMD). AIRE is involved in immune regulation and is known to mediate E3 ubiquitin ligase activity. Defects in AIRE are responsible for the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Specifications

• Antigen: AIRE
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Human
• Gene ID (Entrez): 326
• Primary or Secondary: Primary
• Content And Storage: 4° C

• Clone: TM-724
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: O43918
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.