PeproTech Adiponectin Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17894923	PeproTech 500-P239-50UG	50 μg
17885823	PeproTech 500-P239-100UG	100 μg
17815343	PeproTech 500-P239-1MG	1 mg

Description

AA Sequence of recombinant protein: RGHHHHHHHH ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Adiponectin. Anti-Human Adiponectin-specific antibody was purified by affinity chromatography employing an immobilized Human Adiponectin matrix. Sandwich ELISA: To detect Human Adiponectin by sandwich ELISA (using 100 μL/well) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Adiponectin (500-P239BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human Adiponectin. Western Blot: To detect Human Adiponectin by Western Blot analysi this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adiponectin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.

Specifications

• Antigen: Adiponectin
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: Q15848
• Gene ID (Entrez): 9370
• Immunogen: Hi-5 Insect cells-derived Recombinant Human Adiponectin.
• Purification Method: Antigen affinity chromatography
• Gene: ADIPOQ

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: 30 kDa adipocyte complement-related protein; 30kDa; Acdc; Acrp30; Ad; adipo; adipocyte complement related protein; adipocyte complement related protein of 30 kDa; adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; adiponectin; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADIPQTL1; ADPIOQ; ADPN; Apm1; APM-1; APN; GBP28; Gelatin-binding protein; gelatin-binding protein 28
• Gene Symbols: ADIPOQ
• Primary or Secondary: Primary
• Content And Storage: -20°C