PeproTech Adipolean variant Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  PeproTech 500-P205-50UG

Product Code. 17865223

Description

AA Sequence of recombinant protein: PGAEGPRGFP GIQGRKGEPG EGAYVYRSAF SVGLETYVTI PNMPIRFTKI FYNQQNHYDG STGKFHCNIP GLYYFAYHIT VYMKDVKVSL FKKDKAMLFT YDQYQENNVD QASGSVLLHL EVGDQVWLQV YGEGERNGLY ADNDNDSTFT GFLLYHDTN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human gAcrp30/Adipolean Variant. Anti-Human gAcrp30/Adipolean Variant-specific antibody was purified by affinity chromatography employing an immobilized Human gAcrp30/Adipolean Variant matrix. Sandwich ELISA: To detect Human gAcrp30/Adipolean Variant by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human gAcrp30/Adipolean Variant (500-P205Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human gAcrp30/Adipolean Variant. Western Blot: To detect Human gAcrp30/Adipolean Variant by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human gAcrp30/Adipolean Variant is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

The gAcrp30 variant is a naturally occurring globular protein obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, Acrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and named AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver. This naturally occurring variant of human gAcrp30/Adipolean is an 18.1 kDa protein, containing 14 extra amino acids extra at the N-terminus of human gAcrp30/Adipolean.

Specifications

• Antigen: Adipolean variant
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ADIPOQ
• Gene Alias: gAcrp30
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9370
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q15848
• Gene Symbols: ADIPOQ
• Immunogen: E.coli-derived Recombinant Human gAcrp30/Adipolean Variant.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody