AID, clone: mAID-2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5959-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal mAID-2 recognizes human and mouse AID (gene AICDA) also known as Activation-Induced Cytidine Deaminase. AID is a 24kDa RNA-editing enzyme that converts cytosine into uracil. It plays a critical role during B cell development, specifically in somatic hypermutation (SHM) and immunoglobulin class switch recombination (CSR). Changes in levels of AID expression typically present with disease. For example, mutations in AID are found in Hyper-IgM Syndrome (type 2), a disease form without opportunistic infections, while overexpression correlates with poor prognosis in chronic lymphocytic leukemia/small lymphocytic lymphoma.AID is expressed mainly by activated mature B cells such as mature germinal centre (GC) B cells but also in intermediate GC cells (defined as IgD+CD38-CD23-CD71+). In addition to hematopoietic cells, AID is expressed in primary human hepatocellular carcinomas, helicobacter infected gastric epithelial cells, oocytes and embryonic stem cells although it role in these cells is not well understood. Because AID contains an NLS (nuclear localization signal), expression can be found both in the nucleus as well as in the cytoplasm.Applications Reported: This mAID-2 antibody has been reported for use in immunoblotting (WB), immunohistologic staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This mAID-2 antibody has been tested by western blot and/or immunohistochemistry on FFPE sections using either low or high pH antigen retrieval. This can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Activation-Induced Cytidine Deaminase (AID)|
|PBS with 0.09% sodium azide; pH 7.2|
|ImmunohistochemistryþImmunohistochemistry (Frozen)þWestern Blot|
|Store at 2-8°C.|
For Research Use Only.