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Actin (muscle), Alexa Fluor 488, clone: HHF35, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-6496-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15530237

  • £79.50 / 25µg

Estimated Shipment date
from Supplier 26-07-2018
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Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.The HHF35 antibody recognizes human, mouse, rat, bovine, canine and non-human primate actin.Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This HHF35 antibody has been tested by immunocytochemistry and can be used at less than or equal to 30 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.


Actin (muscle)
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P62739, P68138, Q3ZC07, P62736, P68032, P68133, P62737, P68033, P68134, P68137, P62738, P68035, P68136
actin, actin alpha 1, actin alpha cardiac, actin alpha cardiac 1, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, alpha, cardiac 1, actin, alpha, vascular smooth muscle, actin, aortic smooth muscle, alpha actin 1, alpha actin 2, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin cardiac, alpha-actin-1, alpha-actin-2, alpha-cardiac actin, alpha-sma, alphac-actin, cell growth-inhibiting gene 46 protein, nemaline myopathy type 3, skeletal alpha actin, smooth muscle alpha-actin
Affinity chromatography
BovineþCanineþEquineþGuinea PigþHumanþMurineþNon-human PrimateþPorcineþRat
ImmunocytochemistryþImmunohistochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)
Alexa Fluor 488
ACTA1, Acta2, ACTC1
IgG1, kappa
Store at 2-8°C. Do not freeze. Light-sensitive material.
58, 59, 70

For Research Use Only.